DROSOPHILA S2 CELLS ARE NON-PERMISSIVE FOR VACCINIA VIRUS DNA REPLICATION FOLLOWING ENTRY VIA LOW PH-DEPENDENT ENDOCYTOSIS AND EARLY TRANSCRIPTION.

Drosophila S2 cells are non-permissive for vaccinia virus DNA replication following entry via low pH-dependent endocytosis and early transcription.

Vaccinia virus (VACV), a member of the chordopox subfamily of the Poxviridae, abortively infects insect cells.We have investigated VACV infection of Drosophila S2 cells, which are useful for protein expression and genome-wide RNAi screening.Biochemical and electron microscopic analyses indicated that VACV entry into Drosophila S2 cells depended on

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